Quantitative Optical Imaging

Course description

Quantitative Optical Imaging explores the fundamentals of optical imaging in biology, especially molecular and cellular biology. Covered topics include an introduction to optics and microscopes, fluorescence microscopy and image analysis, and biological applications. MATLAB will be taught at the beginning of the course and used throughout for image processing. Prior experience with MATLAB (or Python) is highly recommended but not required.

Additional details

This course is co-taught by Dr. Joe Dragavon and myself. It is available for students in Biochemistry and MCD Biology, as either an undergraduate course (BCHM/MCDB 4312) or a graduate course (BCHM/MCDB 5312). The graduate version requires students to host a journal club as the graduate-level requirement.

Lab course: We offer an optional one-credit microscopy lab that covers the basics of using the microscopes in the BioFrontiers Advanced Light Microscopy core (widefield, spinning disk, and laser scanning confocal), image analysis, and machine learning. The lab course is not offered every year, so please reach out to let us know if you are interested.

Fall 2022 - The lab course is not being offered this year. Fall 2021 - The lab course was open to all students enrolled in MCDB/BCHM 4312/5312. A new course code was available as MCDB 4100/6440. Note that space in the imaging facility was limited and approximately ten slots were available. Fall 2019 - 2020 - The lab course was only offered to graduate students completing the Interdisciplinary Quantitative Biology graduate program.

If you have questions about the course, please feel free to contact me.

Previous course materials